![]() ![]() Liu, A novel ultrasonic Microdissection technique for biomedicine. Shen, Development and application of ultrasonic surgical instruments. Lin, A micromanipulation system with dynamic force-feedback for automatic batch microinjection. Sun, Automated mouse embryo injection moves toward practical use, Proc. Yanagimachi, Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. Yanagimachi, Intracytoplasmic sperm injection in the mouse. Sato, Efficient injection of bull spermatozoa into oocytes using a piezo-driven pipette. Kuikka, Injection guidance system for cellular microinjections, Int. on Information and Automation, (ICIA) Harbin, China, June, 21–23, 2010 Sun, A new piezo-driven ultrasonic cell microinjection system,” Proc. Cheng, Robotics cell injection system with vision and force control: Towards automatic batch biomanipulation. ![]() ![]() Li, Visual-based Impedance Force Control of Out-of-Plane Cell Injection Systems. Yanagimachi, The use of piezo micromanipulation for intracytoplasmic sperm injection of human oocytes. Chen, A Study of the zona piercing process in piezodriven intracytoplasmic sperm injection. Critser, Vibration study of piezodriven pipettes immersed in viscous liquids. Olgac, New technology for cellular piercing: rotationally oscillating μ-injector, description and validation tests. Olgac, Effect of Mercury Columan on the Microdynamics of the Piezo-Driven Pipettes. Olgac, Microdynamics of the Piezo-Driven Pipette in ICSI. From preliminary experiments of cell injection of a large number of zebrafish embryos ( n = 200), the injector pipette can easily pierce through the cell membrane at a low injection speed and almost no deformation of the cell wall, and with a high success rate(96%) and survival rate(80.7%) This new injection approach shows good potential for precision injection with less damage to the injected cells. Furthermore, ultrasonic vibration micro-dissection (UVM) theory is utilized to analyze the piezo-driven cell injection process, and the source of the lateral oscillations of the injector pipette is investigated. Harmful lateral tip oscillations of the injector pipette are reduced substantially without the use of a mercury column. A small piezo stack is sufficient to perform the cell injection process. This new piezo-driven cell injector design relocates the piezo oscillation actuator to the injector pipette which eliminates the vibration effect on other parts of the micromanipulator. A new design of the piezo-driven cell injector is proposed for automated suspended cell injection. In this paper, a new cell injection system for automatic batch injection of suspended cells is developed. Although this technique significantly improves the survival rates of the ICSI process, shortcomings due to the toxicity of mercury and damage to the cell membrane due to large lateral tip oscillations of the injector pipette may limit its application. Recently a new cell injection technology using piezo-driven pipettes with a very small mercury column was proposed and successfully applied in ICSI to a variety of mammal species. Over the past decade, the rapid development of biotechnologies such as gene injection, in-vitro fertilization, intracytoplasmic sperm injection (ICSI) and drug development have led to great demand for highly automated, high precision equipment for microinjection. ![]()
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